Evaluation of Dual Immunohistochemistry and Chromogenic In Situ Hybridization for HER2 on a Single Section
نویسندگان
چکیده
منابع مشابه
Evaluation of dual immunohistochemistry and chromogenic in situ hybridization for HER2 on a single section.
The evaluation of HER2 status in invasive breast carcinoma can be performed by multiple methods. We assessed the feasibility of performing 2 of these, chromogenic in situ hybridization (CISH) and immunohistochemical staining, on single tissue sections of breast carcinoma. During assay development, sequential performance of immunohistochemical staining after CISH resulted in weaker HER2 expressi...
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Background & Objective: The her2 amplification plays an important role in breast cancer management. Therefore, there is a need for using supplementary molecular methods in IHC equivocal cases. Present study has been conducted to determine the effects of clinicopathological variables on her2 gene amplification by chromogenic in situ hybridization (CISH) in IHC ...
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Trastuzumab is a humanized monoclonal antibody that is approved for the treatment of breast and gastric cancers that overexpress human growth factor receptor 2 (HER2). Since its approval, controversy has existed over whether immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH) is the best method for assessing HER2 status. Proponents of IHC point out that trastuzumab targets t...
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The purpose was to evaluate and compare 5 different HER2 genetic assays with different characteristics that could affect the performance to analyze the human epidermal growth factor 2 (HER2) gene copy number under low and high throughput conditions. The study included 108 tissue samples from breast cancer patients with HER2 immunohistochemistry (IHC) results scored as 0/1+, 2+, and 3+. HER2 gen...
متن کاملDual color chromogenic in situ hybridization for determination of HER2 status in breast cancer: a large comparative study to current state of the art fluorescence in situ hybridization
BACKGROUND Chromogenic in situ hybridization (CISH) is fast becoming a well established technique for easy and sensitive determination of HER2 gene status in breast cancer. However, for the chromogenic method to achieve status as a safe and reliable technique, the method needs to be validated against already known and validated FISH techniques. METHODS Here it is reported from a comparative s...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2012
ISSN: 0002-9173,1943-7722
DOI: 10.1309/ajcplnhinn9o6ysf